Adult tissue homeostasis and repair is regulated by stem cells found both within the tissue and recruited from outside the organ. The timing and mechanism governing niche formation and stem cell specification are not known. Similarly, little is known about the regulation of homing and migration of stem cells to the site of injury. To address these fundamental questions in stem cell biology we utilize the continuously growing incisor tooth model whose continuous growth is supported by lifelong maintenance of epithelial stem cells, and stable influx of mesenchymal stem cells. We combine state of the art genomic, cellular, and molecular biology approaches in order to unravel the mechanisms which regulate these stem cell populations during early development and in postnatal life.
Juuri E, Balic A (2021) Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells.
J Vis Exp. 2021 Jul 8;(173). doi: 10.3791/62462. PMID: 34309587
Krivanek J, Soldatov RA, Kastriti ME, Chontorotzea T, Herdina AN, Petersen J, Szarowska B, Landova M, Matejova VK, Holla LI, Kuchler U, Zdrilic IV, Vijaykumar A, Balic A, Marangoni P, Klein OD, Neves VCM, Yianni V, Sharpe PT, Harkany T, Metscher BD, Bajénoff M, Mina M, Fried K, Kharchenko PV, Adameyko I (2020) Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.
Nat Commun. 2020 Sep 23;11(1):4816. doi: 10.1038/s41467-020-18512-7. PMID: 32968047
Binder M, Biggs LC, Kronenberg MS, Schneider P, Thesleff I, Balic A (2020) Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation.
Sci Rep. 2020 Mar 18;10(1):4963. doi: 10.1038/s41598-020-60708-w. PMID: 32188889
Binder M, Chmielarz P, Mckinnon PJ, Biggs LC, Thesleff I, Balic A (2019) Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche.
Stem Cells. 2019 Sep;37(9):1238-1248. doi: 10.1002/stem.3042. Epub 2019 Jun 10. PMID: 31145830
Järvinen E, Shimomura-Kuroki J, Balic A, Jussila M, Thesleff I (2018) Mesenchymal Wnt/β-catenin signaling limits tooth number.
Development. 2018 Feb 21;145(4):dev158048. doi: 10.1242/dev.158048. PMID: 29437780
Balic A, Aguila HL, Caimano MJ, Francone VP, Mina M (2010) Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars.
Bone. 2010 Jun;46(6):1639-51. doi: 10.1016/j.bone.2010.02.019. Epub 2010 Feb 26. PMID: 20193787